Effect of Soaking Heat Cured Acrylic Resin on Citronella Extract Solution (Cymbopogon nardus) on Streptococcus mutans Colony

Author's Information:

Mirna Febriani

Department of Dental Materials and Technology, Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Poetry Oktanauli

Department of Oral Biology, Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Herlia Nur Istindiah

Department of Orthodontics, Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Fransiska Nuning Kusmawati

Department of Prosthodontics, Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Tuti Alawiyah

Department of Dental Materials and Technology, Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Wilson Christian

Faculty of Dentistry, Prof. Dr. Moestopo (Beragama) University, Jakarta

Vol 05 No 07 (2025):Volume 05 Issue 07 July 2025

Page No.: 171-176

Abstract:

Background: heat cured acrylic resin is a common material for denture bases. This material has disadvantages, such as porosity that cause plaque formation which can cause Denture stomatitis, such as Streptococcus mutans. The common preventive action was cleaning the denture bases with chlorhexidine, but it has side effects. The alternative natural disinfectant that is currently being developed as a soaking agent for heat cured acrylic resin plates, but still has limited research data is citronella. Citronella has antibacterial properties such as essential oils, saponins and flavonoids. Objective: This study aims to explain the effect of immersion the heat cured acrylic resin on the number of Streptocooccus mutans colonies. Methods: Invitro experimental research using a post-test with control group design. Immersion of heat cured acrylic plates in 20% citronella leaf extract solution as the treatment group and immersion in distilled water as the negative control group. Results: The number of Streptococcus mutans colonies on immersion of acrylic plates in a solution of 20% citronella extract and distilled water respectively was 2.1×105 CFU/cm2 and 1.4×106 CFU/cm2. Conclusion: There is an effect of soaking heat-cured acrylic resin in 20% concentration of citronella leaf extract solution on Streptococcus mutans colonies.

KeyWords:

Heat cured acrylic resin, Citronella, Streptococcus mutans

References:

  1. Fahruddin A, Harniati ED, Jayanti LW. A Library Review of Color Change on Heat Cured Acrylic Resin. [Desertation]. Semarang: FKGUMS, 2021:15
  2. Chondro RT, Chaterina DNK, Sari RP. The effectiveness of the addition of hydroxyapatite on the decrease in porosity of the base of heat-cured acrylic resin. Denta J Ked Teeth. 2019; 13(2): 37-42
  3. Savitri RPA, Naini A, Parnaadji R, Kristiana D. Effect of Heat Cured Acrylic Resin Soaking on Tobacco Leaf Extract (Nicotiana tabacum) 50% on Color Change. Padjajaran Journal of Dental Researchers and Students. 2022; 6(3):290-297
  4. Wirayuni KA. Accumulation of Streptococcus mutans on Removable Denture Base of Thermoplastic Nylon Plate and Acrylic Resin. Interdental Journal of Dentistry. 2017; 2(13): 28-31
  5. Oktaria I. Prevention and Management of Denture Stomatitis. Interdental Jurnal Kedokteran Gigi. 2022; 2(18):67-73
  6. Adiguna P, Santoso O. Effect of Lemongrass Leaf Extract (Cymbopogon citratus) on Various Concentrations on the Viability of Streptococcus mutans Bacteria. Journal of Diponegoro Dentistry. 2017; 4(6): 1543-1550
  7. Patricia OMSP, Naliani S, Sugiaman VK. Wibisono JAV. Antibacterial Effectiveness of Red Fruit Extract (Pandanus conoideus Lam) Against S. mutans As An Acrylic Resin Based Denture Cleanser. Makassar Dental Journal. 2023; 1(12):43-48
  8. Ratwita DF. Counseling About Disinfection During Repair of Acrylic Resin Dentures at Dental Laboratories in Malang and Kediri. Darmabakti Cendekia: Journal of Community Service and Engagements. 2020; 2(2):51-57
  9. Rochdjatun I. Diseases in Medicinal Plants, Spices and Stimulants. Malang: UB Press; 2016:73
  10. Rizkita AD. Antibacterial Effectiveness of Fragrant Lemongrass Leaf Extract, Green Betel Leaf, and Red Ginger Against the Growth of Streptococcus mutans. National Seminar on Science and Technology. 2017:1-7
  11. Dewi ZD, Nur A, Hertriani T. Antibacterial effect and inhibition of lemongrass extract biofilm (Cymbopogon nardus L.) against Streptococcus mutans bacteria. Indonesia Dental Magazine. 2015; 2(1):136-141
  12. Mayasari U. Antibacterial Activity Test of Citronella Leaves (Cymbopogon nardus) on the Growth of Streptococcus mutans Bacteria. Journal of Chlorophyll. 2019; 2(3):5-19
  13. Hasibuan SR, Benarivo TG, Cindy AP, Hutagalung MHP, Erawati S. Comparison of the Effectiveness of Lemongrass Extract with Temulawak in Inhibiting the Growth of Streptococcus mutans. Sandi Husada Health Scientific Journal. 2021; 1(10):208-213
  14. Andayani L, Ridawan A, Aryandi R, Salnus S. Antibacterial Effectiveness Test of Lemongrass Leaf Extract on the Growth of Streptococcus mutans. Journal of Medical Laboratory Technology Blood Smear. 2022; 1(3):36-43
  15. Andry. Effect of Avocado Leaf Extract Solution on the Growth of Streptococcus mutants  on Heat Cured Acrylic Resin Denture Plates. [Desertation]. Jakarta: FKGUPDM(B), 2022: 47
  16. Shen C, Rawls HR, Upshaw JFE. Phillips’ Science of Dental Materials, 13th ed. Missouri: Elsevier; 2021:233-252
  17. Terrones-Fernandez I, Casino P, López A, Peiró S, Ríos S, Nardi-Ricart A, et al. Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration. Microbiol Spectr. 2023; 1(11); 1-11
  18. Hafsan. Analytical Microbiology. Makassar: Alauddin University Press. 2014:29; 150-192
  19. Bota W, Martosupono, Rondonuwu F. Potential of Citronella Oil Compounds from Cymbopogon nardus L. as Antibacterial Agents. National Seminar on Science and Technology. January 8, 2015
  20. Anggraini W, Nisa CS, Ramadhani R, Ma'arif B. Antibacterial Activity of Ethanol Extract 96% Cantaloupe Fruit (Cucumis melo L. var. cantalupensis) against the growth  of Escherichia coli bacteria. Pharmaceutical Journal of Indonesia. 2019; 1(5):61-66
  21. Manik DF, Hertiani T, Anshory H. Correlation Analysis Between Flavonoid Levels and Antibacterial Activity of Ethanol Extract and Kersen Leaf Fractions (Muntingia calabura L.) Against Staphylococcus aureus. Khazanah: UII Student Journal. 2014; 2(6):1-11
  22. Saptowo A, Supriningrum R, Supomo. Antibacterial Activity Test of Serefinery Stem Bark Extract (Embeliaborneensis scheff) Against Propionibacterium acnes and Staphylococcus epidermidis. Al Ulum: Journal of Science and Technology. 2022; 7(2):93-97